Short Course: Fundamentals of Mass Spectrometry and Applications of Mass Spectrometry

08:30 – 12:00 | Fundamentals of Mass Spectrometry in the Analysis of Protein Therapeutics

Instructor
Chris Chumsae, Bristol Myers Squibb-Company

This introductory course will provide an overview of the fundamentals of Mass Spectrometry, including the history of Mass Spectrometry, terminology, data interpretation and sample handling. We will include a discussion on modes of operation (parent versus fragment ion analysis), components of a current MS system, and selected applications of Mass Spec as it applies to protein analysis in the biotechnology industry. A discussion on software (basic interpretation and operation) will be presented along with a hardware discussion (strengths for various applications). A limited discussion on small molecule analysis will be presented. 

13:00 – 16:30 | Applications of Mass Spectrometry to Characterize Protein Therapeutics

Instructors
Andy Mahan, Johnson & Johnson
Rich Rogers, Umoja Biopharma

The afternoon section will focus on practical industrial uses of Mass Spectrometry in the analysis of protein therapeutics as well as in Cell and Gene Therapy. The specific topics will be Intact Mass Analysis, Structural MS, Host Cell Protein ID and Peptide Mapping and Multi-Attribute Method and protein post translational modifications: detection and quantitation. The discussions will be driven by industry leaders. Two interactive segments will cover live data analysis demos of both Intact and Reduced Mass Analysis as well as Peptide Mapping Data Analysis in variety of vendors software products. 

This course will review the role MS has in newer therapeutic modalities such as multi-specific, antibody-drug conjugates, cell and gene therapy (AAV, oligonucleotides).  Additionally, there will be sections covering mass spec techniques which evaluate protein structure such as hydroxy radical foot-printing, cross-linking, hydrogen-deuterium exchange, top down and middle down methods and ion mobility. The increasing use of Native MS in the analysis if mis-paired Multispecifics as well as investigation of non-covalent interaction will be covered.  The 2nd part of the afternoon session focuses on Multi-Attribute Method (MAM), MS in QC, PTM quantitation as well as Sequence Variant Analysis and the role of MS in Cell Line Selection.