The advent of ultra-high-pressure LC (UHPLC) and its successful commercialization in the last few years has brought forth a modern HPLC platform capable of higher speed, resolution, precision and sensitivity. Currently, all major HPLC manufacturers have some type of low-dispersion UHPLC products with upper pressure limits ranging from 10,000 to 19,000 psi.
This paper describes a number of popular myths or half-truths in ultra-high-pressure liquid chromatography (UHPLC) and provides data or case studies that delineate, contradict or even repudiate some of these popular beliefs. These are:
- Viscous heating is a huge issue for sub-2um particle columns of inner diameters > 2 mm.
- Binary high-pressure mixing pump is a “must” for a low-dwell volume system.
- One can reduce dwell volume to near zero without performance issues.
- UHPLC provides substantially higher sensitivity than HPLC.
- Method transfer from UHPLC to HPLC is “a piece of cake” and method re-validation is not needed.
- One needs gold plated fittings with double ferrules in UHPLC to withstand the pressure.